pcdna3 1 bira ha (Addgene inc)
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pcdna3 1 bira ha
Pcdna3 1 Bira Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mcs+bira+r118g+ha/pcDNA3%2E1+MCS-BirA(R118G)-HA+(Plasmid+%2336047)/pm41787126-297-4-7
Average 94 stars, based on 101 article reviews
Pcdna3 1 Bira Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mcs+bira+r118g+ha/pcDNA3%2E1+MCS-BirA(R118G)-HA+(Plasmid+%2336047)/pm41787126-297-4-7
Average 94 stars, based on 101 article reviews
pcdna3 1 bira ha - by Bioz Stars,
2026-10
94/100 stars
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Clone Assay:Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis. Article Snippet: Plasmids The WT or mutant FKBP4 was cloned into the pGEM®-T Easy vector (Promega, Madison, WI, USA). pcDNA3.1 MCS-BirA (R118G)-HA was acquired from Addgene #36047. .. The pTA-FKBP4 WT or pTA-FKBP4 F130Y was PCRamplified and cloned into the NheI and AgeI sites of the Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis. Article Snippet: For a 60 mm × 60 mm dish culture, cells were infected with each virus plus DMEMhigh glucose medium containing 0.8 μg/ml polybrene (Millipore, Billerica, MA, USA) for 24 h. The transduced cells were selected with DMEM-high glucose medium containing 1 μg/ml puromycin (Sigma-Aldrich) for 3 days. .. Plasmids The WT or mutant FKBP4 was cloned into the pGEM®-T Easy vector (Promega, Madison, WI, USA). Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis Article Snippet: For a 60 mm × 60 mm dish culture, cells were infected with each virus plus DMEM-high glucose medium containing 0.8 μg/ml polybrene (Millipore) for 24 h. The transduced cells were selected with DMEM-high glucose medium containing 1 μg/ml puromycin (Sigma-Aldrich) for 3 days. .. The WT or mutant FKBP4 was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA). Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis Article Snippet: The WT or mutant FKBP4 was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA). pcDNA3.1 MCS-BirA (R118G)-HA was acquired from Addgene #36047. .. The pTA-FKBP4 WT or pTA-FKBP4 F130Y was PCR-amplified and cloned into the NheI and AgeI sites of the Mutagenesis:Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis. Article Snippet: For a 60 mm × 60 mm dish culture, cells were infected with each virus plus DMEMhigh glucose medium containing 0.8 μg/ml polybrene (Millipore, Billerica, MA, USA) for 24 h. The transduced cells were selected with DMEM-high glucose medium containing 1 μg/ml puromycin (Sigma-Aldrich) for 3 days. .. Plasmids The WT or mutant FKBP4 was cloned into the pGEM®-T Easy vector (Promega, Madison, WI, USA). Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis Article Snippet: For a 60 mm × 60 mm dish culture, cells were infected with each virus plus DMEM-high glucose medium containing 0.8 μg/ml polybrene (Millipore) for 24 h. The transduced cells were selected with DMEM-high glucose medium containing 1 μg/ml puromycin (Sigma-Aldrich) for 3 days. .. The WT or mutant FKBP4 was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA). other:Article Title: HNF1β bookmarking involves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin. Article Snippet: Resource HNF1b bookmarking invol ves Topoisomerase 1 activation and DNA topology relaxation in mitotic chromatin Plasmid Preparation:Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis Article Snippet: For a 60 mm × 60 mm dish culture, cells were infected with each virus plus DMEM-high glucose medium containing 0.8 μg/ml polybrene (Millipore) for 24 h. The transduced cells were selected with DMEM-high glucose medium containing 1 μg/ml puromycin (Sigma-Aldrich) for 3 days. .. The WT or mutant FKBP4 was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA). Article Title: Sensitivity to Vγ9Vδ2TCR T cells is imprinted after single mutations during early oncogenesis Article Snippet: .. Article Title: Scribble maintains angiogenic sprout integrity through control of cortical actomyosin Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. mEmerald-MyosinIIB was a gift from M. Davidson’s lab. For the VE-cadherin-⍺-catenin-mEmerald chimera fusion protein, fragments of mouse VE-cadherin and ⍺-catenin were isolated from mouse cDNA library, and mEmerald was a gift from M. Davidson’s lab. For LifeAct-mScarlet, LifeAct was isolated from LifeAct-GFP (plasmid # 51010, Addgene), and mScarlet was a gift from Y. Liu. .. For VE-cadherinBioID-HA and BioID-HA, human VE-cadherin was a gift from M. Davidson’s lab, and BioID-HA was isolated from Polymerase Chain Reaction:Article Title: Hsp90 co-chaperone FKBP4 facilitates CCT8 folding and connects Hsp90 to chaperonin-dependent proteostasis Article Snippet: The WT or mutant FKBP4 was cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA). pcDNA3.1 MCS-BirA (R118G)-HA was acquired from Addgene #36047. .. The pTA-FKBP4 WT or pTA-FKBP4 F130Y was PCR-amplified and cloned into the NheI and AgeI sites of the Isolation:Article Title: Scribble maintains angiogenic sprout integrity through control of cortical actomyosin Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. mEmerald-MyosinIIB was a gift from M. Davidson’s lab. For the VE-cadherin-⍺-catenin-mEmerald chimera fusion protein, fragments of mouse VE-cadherin and ⍺-catenin were isolated from mouse cDNA library, and mEmerald was a gift from M. Davidson’s lab. For LifeAct-mScarlet, LifeAct was isolated from LifeAct-GFP (plasmid # 51010, Addgene), and mScarlet was a gift from Y. Liu. .. For VE-cadherinBioID-HA and BioID-HA, human VE-cadherin was a gift from M. Davidson’s lab, and BioID-HA was isolated from Transfection:Article Title: Scribble maintains angiogenic sprout integrity through control of cortical actomyosin Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. mEmerald-MyosinIIB was a gift from M. Davidson’s lab. For the VE-cadherin-⍺-catenin-mEmerald chimera fusion protein, fragments of mouse VE-cadherin and ⍺-catenin were isolated from mouse cDNA library, and mEmerald was a gift from M. Davidson’s lab. For LifeAct-mScarlet, LifeAct was isolated from LifeAct-GFP (plasmid # 51010, Addgene), and mScarlet was a gift from Y. Liu. .. For VE-cadherinBioID-HA and BioID-HA, human VE-cadherin was a gift from M. Davidson’s lab, and BioID-HA was isolated from |